摘要：目的探討外源性干擾素(interferon,IFN)-β對(duì)人類少突膠質(zhì)細(xì)胞(oligodendrocyte,OL)和博爾納病病毒(Borna disease virus,BDV)持續(xù)感染的OL細(xì)胞(OL/BDV)中內(nèi)源性Ⅰ型IFN表達(dá)和干擾素調(diào)節(jié)因子(interferon regulatory factor,IRF)7細(xì)胞定位的影響。方法外源性IFN-β處理OL細(xì)胞和OL/BDV細(xì)胞0、0.5、2、4、8、24 h,根據(jù)細(xì)胞是否加入外源性IFN-β,分為外源性IFN-β未刺激組和刺激組。采用實(shí)時(shí)熒光定量PCR(quantitative real-time PCR,qPCR)檢測(cè)Ⅰ型IFN mRNA表達(dá)。OL細(xì)胞和OL/BDV細(xì)胞分別轉(zhuǎn)染IRF7-EGFP質(zhì)粒,外源性IFN-β刺激4 h,觀察IRF7細(xì)胞定位情況。結(jié)果外源性IFN-β刺激OL細(xì)胞,在2、4、24 h,IFN-αmRNA表達(dá)顯著增加(P值分別為0.008,0.0001,0.004);在0.5、4、8、24 h,IFN-βmRNA表達(dá)顯著增加(P值分別為0.0004,0.0002,0.011,0.012),兩者均在4 h增加最為顯著。外源性IFN-β刺激OL/BDV細(xì)胞4 h和24 h,IFN-αmRNA表達(dá)顯著增加(P值分別為0.0004,0.022),IFN-βmRNA表達(dá)僅在刺激4增加(P=0.002)。外源性IFN-β刺激OL細(xì)胞和OL/BDV細(xì)胞4 h,IRF7綠色熒光蛋白均轉(zhuǎn)移至細(xì)胞核內(nèi)。結(jié)論外源性IFN-β可誘導(dǎo)OL細(xì)胞和OL/BDV細(xì)胞中內(nèi)源性I型IFN表達(dá),參與解除BDV對(duì)I型IFN應(yīng)答的抑制過(guò)程,并促進(jìn)IRF7的活化入核。